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Registro Completo |
Biblioteca(s): |
Embrapa Amazônia Oriental. |
Data corrente: |
03/10/2018 |
Data da última atualização: |
03/10/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
LIMA, A. M.; CUNHA, E. F. M.; ISHIDA, A. K. N.; PEREIRA, A. C. da C.; REIS, S. P. dos; SOUZA, C. R. B. de. |
Afiliação: |
Aline Medeiros Lima, UFRA/UFPA; ELISA FERREIRA MOURA CUNHA, CPATU; ALESSANDRA KEIKO NAKASONE ISHIDA, CPATU; Angelo Cleiton da Costa Pereira, UFRA/UFPA; Sávio Pinho dos Reis, UFPA/UEPA; Cláudia Regina Batista de Souza, UFPA. |
Título: |
Expression profiles of defense genes in cassava storage roots upon exposure to Phytopythium sp., causal agent of soft root rot disease. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Physiological and Molecular Plant Pathology, v. 104, p. 23-30, Dec. 2018. |
DOI: |
10.1016/j.pmpp.2018.09.001 |
Idioma: |
Inglês |
Conteúdo: |
Cassava is one of the most important crops in Africa, Asia and Latin America, where millions of people depend on its starchy storage roots as their main source of energy. In Brazil, cassava is cultivated in all regions; however, soft root rot disease has hampered its production mainly in the Amazon region. The identification of Phytopythium sp., as the causal agent of soft root rot disease in cassava plants collected in Brazil, was recently reported. Here, our main aim was to evaluate changes in expression levels of genes possibly involved in compatible cassava- Phytopythium sp. interaction. Semi-quantitative RT-PCR assays were performed to evaluate the expression levels of genes coding for proteins with known functions in plant defense, such as enzymes involved in oxidative burst and the phenylpropanoid pathway, in detached cassava storage roots inoculated and noninoculated with pathogen. Our results showed that cassava responded to pathogen infection through up-regulation of genes coding for superoxide dismutase, phenylalanine ammonia-lyase, chalcone synthase, cysteine proteinase inhibitor and RING zinc finger protein. Furthermore, the expression pattern of the allergenic-related glutamic acid-rich protein and translationally controlled tumor protein genes in detached roots used here validated the absence of post-harvest physiological deterioration, a known endogenous disorder affecting the storage roots of cassava at 24?72 h after harvest. This is the first study of interaction between cassava and Phytopythium sp. at the molecular level contributing to understanding how cassava responds to pathogen infection, as well as to future strategies of molecular breeding regarding the tolerance to soft root rot disease. MenosCassava is one of the most important crops in Africa, Asia and Latin America, where millions of people depend on its starchy storage roots as their main source of energy. In Brazil, cassava is cultivated in all regions; however, soft root rot disease has hampered its production mainly in the Amazon region. The identification of Phytopythium sp., as the causal agent of soft root rot disease in cassava plants collected in Brazil, was recently reported. Here, our main aim was to evaluate changes in expression levels of genes possibly involved in compatible cassava- Phytopythium sp. interaction. Semi-quantitative RT-PCR assays were performed to evaluate the expression levels of genes coding for proteins with known functions in plant defense, such as enzymes involved in oxidative burst and the phenylpropanoid pathway, in detached cassava storage roots inoculated and noninoculated with pathogen. Our results showed that cassava responded to pathogen infection through up-regulation of genes coding for superoxide dismutase, phenylalanine ammonia-lyase, chalcone synthase, cysteine proteinase inhibitor and RING zinc finger protein. Furthermore, the expression pattern of the allergenic-related glutamic acid-rich protein and translationally controlled tumor protein genes in detached roots used here validated the absence of post-harvest physiological deterioration, a known endogenous disorder affecting the storage roots of cassava at 24?72 h after harvest. This is the first study of inter... Mostrar Tudo |
Thesagro: |
Defesa Vegetal; Doença; Doença de Planta; Mandioca; Podridão Radicular. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 02563naa a2200253 a 4500 001 2096813 005 2018-10-03 008 2018 bl uuuu u00u1 u #d 024 7 $a10.1016/j.pmpp.2018.09.001$2DOI 100 1 $aLIMA, A. M. 245 $aExpression profiles of defense genes in cassava storage roots upon exposure to Phytopythium sp., causal agent of soft root rot disease.$h[electronic resource] 260 $c2018 520 $aCassava is one of the most important crops in Africa, Asia and Latin America, where millions of people depend on its starchy storage roots as their main source of energy. In Brazil, cassava is cultivated in all regions; however, soft root rot disease has hampered its production mainly in the Amazon region. The identification of Phytopythium sp., as the causal agent of soft root rot disease in cassava plants collected in Brazil, was recently reported. Here, our main aim was to evaluate changes in expression levels of genes possibly involved in compatible cassava- Phytopythium sp. interaction. Semi-quantitative RT-PCR assays were performed to evaluate the expression levels of genes coding for proteins with known functions in plant defense, such as enzymes involved in oxidative burst and the phenylpropanoid pathway, in detached cassava storage roots inoculated and noninoculated with pathogen. Our results showed that cassava responded to pathogen infection through up-regulation of genes coding for superoxide dismutase, phenylalanine ammonia-lyase, chalcone synthase, cysteine proteinase inhibitor and RING zinc finger protein. Furthermore, the expression pattern of the allergenic-related glutamic acid-rich protein and translationally controlled tumor protein genes in detached roots used here validated the absence of post-harvest physiological deterioration, a known endogenous disorder affecting the storage roots of cassava at 24?72 h after harvest. This is the first study of interaction between cassava and Phytopythium sp. at the molecular level contributing to understanding how cassava responds to pathogen infection, as well as to future strategies of molecular breeding regarding the tolerance to soft root rot disease. 650 $aDefesa Vegetal 650 $aDoença 650 $aDoença de Planta 650 $aMandioca 650 $aPodridão Radicular 700 1 $aCUNHA, E. F. M. 700 1 $aISHIDA, A. K. N. 700 1 $aPEREIRA, A. C. da C. 700 1 $aREIS, S. P. dos 700 1 $aSOUZA, C. R. B. de 773 $tPhysiological and Molecular Plant Pathology$gv. 104, p. 23-30, Dec. 2018.
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Embrapa Amazônia Oriental (CPATU) |
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Registro Completo
Biblioteca(s): |
Embrapa Amapá. |
Data corrente: |
10/11/2023 |
Data da última atualização: |
10/11/2023 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
NUNES, J. C. G.; EULER, A. M. C.; FREITAS, T. P. M. de. |
Afiliação: |
JULIANA CHAGAS GURJÃO NUNES, UNIVERSIDADE FEDERAL DO AMAPÁ; ANA MARGARIDA CASTRO EULER, CPAF-AP; TATIANA PARÁ MONTEIRO DE FREITAS, INSTITUTO FEDERAL DE EDUCAÇÃO, CIÊNCIA E TECNOLOGIA DO PARÁ. |
Título: |
Mapeamento de açaizais nativos com uso de drone na comunidade Arraiol do Bailique, AP, Brasil. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
In: JORNADA CIENTÍFICA DA EMBRAPA AMAPÁ, 7., 2021, Macapá. Transversalidade da ciência, tecnologia e inovações para o planeta: resumos. Macapá: Embrapa Amapá, 2022. |
Páginas: |
p. 19. |
Idioma: |
Português |
Notas: |
Editoras técnicas: Cristiane Ramos de Jesus, Ana Cláudia Lira-Guedes e Adelina do Socorro Serrão Belém. ODS-15. |
Conteúdo: |
O objetivo da pesquisa foi realizar o mapeamento de açaizais nativos em uma comunidade produtora de açaí do território do Bailique e levantar informações sobre a sua distribuição espacial a partir de imagens de alta resolução espacial oriundas de veículo aéreo não tripulado (VANT), popularmente conhecido como drone. |
Palavras-Chave: |
Classificação automática; Interpretação visual; Mapeamento; Object-Based Image Analysis (OBIA); Objetivo de Desenvolvimento Sustentável 15. |
Thesagro: |
Açaí; Euterpe Oleracea; Várzea. |
Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1158168/1/Mapeamento-de-acaizais.pdf
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Marc: |
LEADER 01349nam a2200253 a 4500 001 2158168 005 2023-11-10 008 2022 bl uuuu u00u1 u #d 100 1 $aNUNES, J. C. G. 245 $aMapeamento de açaizais nativos com uso de drone na comunidade Arraiol do Bailique, AP, Brasil.$h[electronic resource] 260 $aIn: JORNADA CIENTÍFICA DA EMBRAPA AMAPÁ, 7., 2021, Macapá. Transversalidade da ciência, tecnologia e inovações para o planeta: resumos. Macapá: Embrapa Amapá$c2022 300 $ap. 19. 500 $aEditoras técnicas: Cristiane Ramos de Jesus, Ana Cláudia Lira-Guedes e Adelina do Socorro Serrão Belém. ODS-15. 520 $aO objetivo da pesquisa foi realizar o mapeamento de açaizais nativos em uma comunidade produtora de açaí do território do Bailique e levantar informações sobre a sua distribuição espacial a partir de imagens de alta resolução espacial oriundas de veículo aéreo não tripulado (VANT), popularmente conhecido como drone. 650 $aAçaí 650 $aEuterpe Oleracea 650 $aVárzea 653 $aClassificação automática 653 $aInterpretação visual 653 $aMapeamento 653 $aObject-Based Image Analysis (OBIA) 653 $aObjetivo de Desenvolvimento Sustentável 15 700 1 $aEULER, A. M. C. 700 1 $aFREITAS, T. P. M. de
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